Use of Different I181-labelled Antibody Eluates in the Characterization of Shigella and Vibrio Antisera

Agglutinating Efficiency and Combining Capacity of Shigella and Vibrio Antisera from Rabbits at Different Stages of Immunization

Studies of the relative combining capacities of different antisera to Vibrio cholerae and Shigella flexneri were carried out using the Talmage test. In this test the absorption of an I(131) labelled antibody preparation by antigen is blocked by the addition of unlabelled unknown serum. Sera from rabbits in the early stages of immunization ("early antisera") had a high agglutinin titer, but low ...

RAPID IDENTIFICATION OF Vibrio cholera-01 BY COAGGLUTINATION TEST USING MONO-SPECIFIC ANTIBODY

Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) ...

The first report of Clostridium oedematiens infection in sheep in Iran by the use of Fluorescent Labelled Antibody

Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera

 Background and purpose: Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and nanostructures have been used. This study aimed at synthesis of gold nanoparticles by apoferriti...

Advantages of Placenta as a Source for Mass Production of HLA-Antisera

Human Leukocyte Antigens (HLA) of fetus are responsible for induction of HLA antibodies in mother. Exposure to the mismatched paternal HLA antigens in the fetus gives rise to a polyclonal antibody response against paternal HLA antigens. HLA antibodies are present in the maternal circulation of the placenta and are absorbed onto the placenta [1]. The major source of HLA antisera is from sera of ...